Studies on chemical constituents of hairy root of Cassia obtusifolia / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the chemical constituents in hairy roots of Cassia obtusifolia.</p><p><b>METHOD</b>The hairy roots of C. obtusifolia were induced with Agrobacterium rhizogenes LBA9402 from cotyledons and cultured in MSO liquid medium. The compounds were isolated by silica gel, polyamide and Sephadex LH-20 column chromatography, and the structures were elucidated by employing chemical and spectral methods</p><p><b>RESULT</b>Eight compounds were isolated from the ethyl acetate fraction of 95% EtOH extract of the transformed roots of C. obtusifolia. They are betulinic acid, chrysophanol, physcion, stigmasterol, 1-hydroxy-7-methoxy-3-methyl-anthraquinone, 8-O-methylchrysophanol, 1-O-methylchrysophanol and aloe-emodin, and aloe-emodin was isolated from the hairy roots of C. obtusifolia for the first time.</p><p><b>CONCLUSION</b>The hairy roots of C. obtusifolia have the ability to synthesize the similar chemical constituents as the original plants.</p>