Screening of high lipid production Trichosporon fermentans mutants by transposon tagging mTn-lacZ/leu2 insertion / 生物工程学报
Chinese Journal of Biotechnology
; (12): 468-474, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-351511
Biblioteca responsável:
WPRO
ABSTRACT
To improve microbial lipid production, we inserted mTn-lacZ/leu2 into Trichosporon fermentans 2.1368-Leu(-) to obtain high lipid production mutants. By observing the LacZ chromogenic change, the positive reaction between Cerulenin (inhibitor of fatty acid synthase) and phosphate vanillin, a higher lipid-producing mutant 2.1368-Leu(-)-7 grown on corn-stalk hydrolysate was obtained. The lipid content of this mutant reached 38.30% (8.97% higher than that of the control) and the lipid production rate was 8.35% (20.63% higher than that of the control). The rate of sugar utilization was 77%, meaning that 100 g corn-stalk could be converted to 8.32 g lipid. The study provided an effective method for microbial lipid production by using cheap raw materials for biodiesel.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Trichosporon
/
Elementos de DNA Transponíveis
/
Mutagênese Insercional
/
Caules de Planta
/
Zea mays
/
Proteínas de Saccharomyces cerevisiae
/
3-Isopropilmalato Desidrogenase
/
Biocombustíveis
/
Fermentação
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2011
Tipo de documento:
Artigo