Mechanism of DNA transformation based on mineral nanofibers and method improvement / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1379-1384, 2010.
Article
em Zh
| WPRIM
| ID: wpr-351583
Biblioteca responsável:
WPRO
ABSTRACT
Sepiolite--an inexpensive, resourceful, fibrous yet inoffensive mineral--made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4 degrees C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Transformação Bacteriana
/
DNA Bacteriano
/
Química
/
Silicatos de Magnésio
/
Eletroporação
/
Fibras Minerais
/
Nanofibras
/
Genética
/
Métodos
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Article