Determination of saponin I in extracts of Allium macrostemnon / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon.</p><p><b>METHOD</b>The extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID.</p><p><b>RESULT</b>The calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%).</p><p><b>CONCLUSION</b>The method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.</p>