Protein profiles of serum in gastric cancer at a high-risk area by SELDI-TOF mass spectrometry / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 677-680, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-352413
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the specific and sensitive biomarkers for gastric cancer detection, a surface-enhanced laser desorption and ionization protein chip mass spectrometry (SELDI-TOF-MS) was used to generate protein profiles of serum in gastric cancer at a high-risk area.</p><p><b>METHODS</b>A total of 36 gastric cancer cases and 46 subjects with superficial gastritis were selected from Linqu county, Shandong province, a high-risk area of gastric cancer. Serum samples were collected and Q10 protein chips were used to detect the serum proteomic patterns, and the sensitivity and specificity were assessed.</p><p><b>RESULTS</b>For the comparison of the gastric cancer group (26 out of 36 gastric cancer cases) versus superficial gastritis group (37 out of 46 subjects), the 6 most discriminating peaks (m/z 8587, 6945, 8243, 3899, 7035, and 9943) were identified by the ProteinChip Data Analysis System (ZUCIPDAS). The sensitivity and specificity of this pattern were 88.5% and 97.3%, respectively. A total of 19 subjects (10 gastric cancer cases and 9 superficial gastritis subjects) was selected to test the accuracy of this pattern by using blind method, and the sensitivity and specificity were 80.0% and 88.9% ,respectively.</p><p><b>CONCLUSION</b>Our findings suggest that SELDI profiling of serum might be a potential for gastric cancer detection and screening in high-risk population.</p>
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Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Doenças do Sistema Digestório
/
Neoplasia do Estômago
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espectrometria de Massas
/
Neoplasias Gástricas
/
Sangue
/
Mapeamento de Peptídeos
/
Proteínas Sanguíneas
/
Biomarcadores Tumorais
/
Estudos de Casos e Controles
/
China
/
Epidemiologia
/
Estudos de Coortes
Tipo de estudo:
Estudo diagnóstico
/
Estudo de etiologia
/
Estudo de incidência
/
Estudo observacional
/
Estudo prognóstico
/
Fatores de risco
Limite:
Idoso
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo