Identification and validation of novel C/EBPbeta-regulated genes in preadipocyte proliferation / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1190-1194, 2010.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-352594
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>CCAAT/enhancer-binding protein beta (C/EBPbeta) is required for mitotic clonal expansion (MCE) during adipogenesis. It is still unclear how C/EBPbeta regulates MCE in the earlier differentiation programs of 3T3-L1 preadipocytes. The purpose of this paper was to understand why C/EBPbeta is required for preadipocyte proliferation, and identify new target genes of C/EBPbeta with chromatin immunoprecipitation (ChIP)-on-chip.</p><p><b>METHODS</b>Postconfluent growth-arrested 3T3-L1 preadipocytes were induced to differentiation using a standard differentiation protocol. ChIP was performed at 20 hours after induction with specific anti-C/EBPbeta antibodies. The precipitated DNA was amplified, labeled and hybridized with a mouse promoter microarray. Compared with the control in which the ChIP experiment was performed with non-specific antibody, only the genes with a signal increasing more than 2 fold were considered as candidate genes.</p><p><b>RESULTS</b>A total of 110 candidate genes were identified. BTG3 associated nuclear protein (SMAR1, Banp) and tripartite motif-containing 35 (Hls5, trim35) were two target genes among the 110 candidate genes which are involved in cell cycle regulation; the binding of C/EBPbeta to the promoter of banp and trim35 was verified by ChIP-PCR.</p><p><b>CONCLUSION</b>C/EBPbeta may regulate preadipocyte proliferation through activation of banp and trim35.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Ligação Proteica
/
Proteínas Nucleares
/
Diferenciação Celular
/
Reação em Cadeia da Polimerase
/
Adipócitos
/
Proteínas de Ciclo Celular
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Proteína beta Intensificadora de Ligação a CCAAT
/
Biologia Celular
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2010
Tipo de documento:
Artigo