Opposite modulatory effects of substance P on GABA-and 5-HT-activated currents in the same sensory neurons / 生理学报
Acta Physiologica Sinica
; (6): 703-707, 2004.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-352711
Biblioteca responsável:
WPRO
ABSTRACT
The modulation by substance P of gamma-aminobutyric acid (GABA)- and 5-hydroxytryptamine (5-HT)-activated currents (I(GABA) and I(5-HT)) was studied by using patch-clamp technique in rat trigeminal ganglion (TG) neurons. The majority of neurons examined responded to GABA and 5-HT with inward currents in the same cells (63.8%, 30/47). In 22 out of 30 neurons sensitive to both GABA and 5-HT, pretreatment with substance P (SP, 0.01 micromol/L) suppressed I(GABA) by (35.7 +/-6.1)% and enhanced I(5-HT) by (65.2 +/- 8.7)%. GR 82334, a potent and specific antagonist of NK1 tachykinin receptor, reversibly blocked the modulatory effects of SP. The SP modulation on I(GABA) and I(5-HT) was also abolished by intracellular dialysis of GDP-beta-S, a non-hydrolyzable GDP analog, or GF 109203X, a selective protein kinase C inhibitor. These results suggest that SP exerts opposite modulatory actions on GABA(A) receptor and 5-HT3 receptor activity of the same primary sensory neuron via the same intracellular signal transduction pathway.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Antagonistas da Serotonina
/
Serotonina
/
Substância P
/
Gânglio Trigeminal
/
Ratos Sprague-Dawley
/
Técnicas de Patch-Clamp
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Antagonistas GABAérgicos
/
Ácido gama-Aminobutírico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo