Effects of extracts of Dragon's blood on fibroblast proliferation and extracellular matrix hyaluronic acid / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 53-57, 2015.
Article
em Zh
| WPRIM
| ID: wpr-353203
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Dragon' s blood extract on proliferation and secret extracellular matrix function of fibroblasts in vitro.</p><p><b>METHODS</b>Dragon' s blood was extracted by chloroform, acetoacetic ester, alcohol. Human fibroblast were cultured in vitro in media containing gradient dilutions of Dragon' s blood extracts (0.002, 0.02, 0.2, 2, 20 mg/ml) , which was followed by cell proliferation assessed with MTT assay on 0, 12, 24, 36, 48, 60, 72 h. Under the optimal concentration, the cell growth curves were drawn and the flow cytometry (FCM) was used to determine the changes of cell cycle. On 0, 12, 24, 36, 48, 60, 72 h, the concentration of hyaluronic acid in the supernatant of fibroblast culture was measured by radioimmunoassay.</p><p><b>RESULTS</b>0.2-2 mg/ml Dragon' s blood extracts enhanced the proliferation of fibroblasts in a dose-dependent manner. 2 mg/ml was the optimal dilution of Dragon's blood extract, and it increased the ratio of S cells in cell cycle [(25.80 ± 3.10)%] than control group [(7.50 ± 0.70)%, P < 0.01]. From 12 h to 72 h, in 2 mg/ml Dragon's blood group, concentration of Hyaluronic acid secreted by fibroblasts gradually increased, but were less than control (P < 0.01).</p><p><b>CONCLUSIONS</b>Dragon's blood acetoacetic ester extract improved the proliferation of cultured human fibroblasts in vitro, might be beneficial to promote wound healing.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Resinas Vegetais
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Fatores de Tempo
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Extratos Vegetais
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Ciclo Celular
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Química
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Secreções Corporais
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Meios de Cultura
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Biologia Celular
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Proliferação de Células
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2015
Tipo de documento:
Article