Establishment of two-dimensional electrophoresis of uterine leiomyoma for the proteome analysis / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.</p><p><b>METHODS</b>Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.</p><p><b>RESULTS</b>The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.</p><p><b>CONCLUSION</b>With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.</p>