Characterization of human metapneumovirus from pediatric patients with acute respiratory infections in a 4-year period in Beijing, China / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1623-1628, 2011.
Article
em En
| WPRIM
| ID: wpr-353995
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Human metapneumovirus (hMPV) was discovered by scientists in the Netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections (ARTI) in China. The objective of this study was to determine the importance of hMPV infection in children in Beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period.</p><p><b>METHODS</b>Clinical specimens collected from children with ARTI from January 2006 to December 2009 were tested for hMPV by RT-PCR using primers targeting the matrix (M) gene, followed by genotyping of hMPV directly from positive samples by diplex PCR with primers for glycoprotein (G) genes. Sequence analysis was used for genotyping of those un-typable samples. Common respiratory viruses in these clinical specimens were tested by virus isolation and antigen detection, in addition to hMPV detection.</p><p><b>RESULTS</b>Of 4730 tested specimens, 191 (4.0%) were positive for hMPV and 62.8% of 191 were identified as genotype A. The positive rate of hMPV from hospitalized patients was higher than that from outpatients each year. Most of hMPV positive children were under five years old. The peak of hMPV activity mostly occurred in late spring and overlapped with or followed that of respiratory syncytial virus (RSV) and followed by parainfluenza virus 3. Of hMPV infected cases, 68.6% were lower respiratory tract infection, among which 79.4% were hospitalized, and upper respiratory tract infection was diagnosed for 31.4% of hMPV infected children. The 9.4% of hMPV positive samples were found to co-exist with other respiratory viruses.</p><p><b>CONCLUSIONS</b>hMPV was an important pathogen for ARTI in pediatric patients, especially those under five years old. Both genotypes A and B circulated simultaneously in Beijing.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Infecções Respiratórias
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Virologia
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Virulência
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China
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Metapneumovirus
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Genética
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Genótipo
Limite:
Adolescent
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Female
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Humans
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Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2011
Tipo de documento:
Article