Production of polyclonal antibody against HBD-2 by immunization with recombinant GST-HBD-2 fusion protein / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 575-579, 2005.
Article
em Zh
| WPRIM
| ID: wpr-354247
Biblioteca responsável:
WPRO
ABSTRACT
For the purpose of detecting the HBD-2 expression at protein level, the recombinant prokaryotic expression vector pGEX-1lambdaT-HBD-2 was constructed and the E. coli-based product of GST-HBD-2 fusion protein was prepared. When rabbit was immunized with the fusion protein, the anti-serum against HBD-2 was produced. After caprylic acid and ammonium sulfate precipitation, high titer of specific polyclonal antibody against HBD-2, which was detected by ELISA and Western blot, was obtained. This result suggests that recombinant peptide fusion protein could be used instead of the conjugate of peptide-albumin or peptide-thyroid globulin to produce antibody. The obtained antibodies could be used for revealing the tissue distribution of HBD-2 and the regulation of its gene expression.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas Recombinantes de Fusão
/
Ensaio de Imunoadsorção Enzimática
/
Western Blotting
/
Imunização
/
Beta-Defensinas
/
Alergia e Imunologia
/
Escherichia coli
/
Metabolismo
/
Anticorpos
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2005
Tipo de documento:
Article