Construction of recombinant baculovirus co-expressing M1 and HA of influenza A virus / 病毒学报
Chinese Journal of Virology
; (6): 231-236, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-354742
Biblioteca responsável:
WPRO
ABSTRACT
The M1 and HA genes of H1N1 influenza virus were amplified and then cloned into the pFastBac dual donor plasmid. The recombinant pFastBac Dual-M1-HA was identified by restriction enzyme digestion. After the pFastBacdual-M1-HA was transformed into the baculovirus shuttle plasmid (bacmid) in DH10Bac competent cells, the colonies were identified by antibiotics and blue-white selection. The rBac-mid-M1-HA was verified by PCR and transfected into S f9 cells to produce recombinant baculovirus (rBac-M1-HA). Gene insertion of rBac-M1-HA was verified and the expression of M1 and HA genes was analyzed by IFA and Western-blot, demonstrating M1 and HA were co-expressed successfully. This study provides the foundation for researching the formation mechanism of influenza VLP and developing new influenza vaccines.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Expressão Gênica
/
Linhagem Celular
/
Proteínas da Matriz Viral
/
Baculoviridae
/
Clonagem Molecular
/
Spodoptera
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
/
Alergia e Imunologia
/
Vírus da Influenza A Subtipo H1N1
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo