In vitro evaluation on the effects of combined lamivudine (3TC) and Ara-AMP against hepatitis B virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 16-19, 2002.
Article
em Zh
| WPRIM
| ID: wpr-355137
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To evaluate the inhibitive effect of combination of 3TC with Ara-AMP against HBV in vitro.</p><p><b>METHODS</b>Used 2.2.15 cell as target cell. With radioimmunological technique and blot slot, the inhibitive effect of 3TC, Ara-AMP and the combination of both against the HBsAg, HBeAg and intracellular HBV DNA were investigated.</p><p><b>RESULTS</b>The inhibitive ratio of Ara-AMP against HBsAg, HBeAg was 45.48% and 41.46% respectively when its concentration was 400.0 microgram/ml. Although 3TC also has inhibitive effect in its experimental concentration, its effect is weaker. When Ara-AMP 50.0 microgram/ml was combined with 3TC 1.25 and 5.00 microgram/ml respectively, the inhibitive ratio against HBsAg were 19.92% and 17.32% respectively. Compared with using same concentration 3TC alone, the difference of results was significant (P<0.05). But when compared with using the same concentration Ara-AMP alone, the difference of results had no statistical significance (P <0.05). Remarkable inhibitive effects of combination of 3TC with Ara-AMP against HBeAg were n ot found. When 3TC 5.00 microgram/ml was combined with Ara-AMP 12.5 and 50.0 microgram/ml respectively, the inhibitive ratio against HBV DNA was 45.90% and 50.36% respectively. Comparing the content of HBV DNA in these groups with that of control group and the groups using the same concentration 3TC and Ara-AMP alone, the differences were significant (P <0.05).</p><p><b>CONCLUSIONS</b>Combination of 3TC with Ara-AMP could enhance the inhibitive effects against HBV DNA.</p>
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Base de dados:
WPRIM
Assunto principal:
Antivirais
/
Farmacologia
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Fosfato de Vidarabina
/
Técnicas In Vitro
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DNA Viral
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Vírus da Hepatite B
/
Lamivudina
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Relação Dose-Resposta a Droga
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Sinergismo Farmacológico
/
Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2002
Tipo de documento:
Article