Regulatory T cells in the treatment of autoimmune myositis in mice: efficacy and mechanism / 南方医科大学学报
Journal of Southern Medical University
; (12): 602-605, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-355318
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effect of CD4(+) CD25(+) Foxp3(+) Tregs in the treatment of autoimmune myositis (EAM) in mice and explore the possible mechanisms.</p><p><b>METHODS</b>Mouse models of EAM were divided randomly into model group and treatment group, and the latter received infusion of CD4(+) CD25(+) Foxp3(+) Tregs separated from normal mouse spleen by magnetic activated cell sorting. The changes of muscle pathology was observed, and the expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs was analyzed using flow cytometry; peripheral blood IL-10 and TGF-β levels were tested using double antibody sandwich ELISA.</p><p><b>RESULTS</b>Compare with the model group, the mice in the treatment group showed significantly reduced muscular inflammatory cell infiltration, increased blood levels of IL-10 and TGF-β (P<0.05), and increased expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs (P<0.05).</p><p><b>CONCLUSION</b>CD4(+) CD25(+) Foxp3(+) Tregs reinfusion produces therapeutic effect in mice with EAM by increasing peripheral blood IL-10 and TGF-β levels and PD-1 and CTLA-4 expressions in spleen CD4(+) CD25(+) Foxp3(+) Tregs.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Doenças Autoimunes
/
Baço
/
Sangue
/
Separação Celular
/
Interleucina-10
/
Linfócitos T Reguladores
/
Modelos Animais de Doenças
/
Alergia e Imunologia
/
Fator de Crescimento Transformador beta1
/
Antígeno CTLA-4
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Artigo