Effect of resveratrol on constrictions of human intrapulmonary arteries ex vivo / 南方医科大学学报
Journal of Southern Medical University
; (12): 540-543, 2015.
Article
em Zh
| WPRIM
| ID: wpr-355332
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effect of resveratrol on constrictions of isolated human intrapulmonary arteries and its mechanisms.</p><p><b>METHODS</b>Intrapulmonary arteries (1-1.5 mm in diameter) were dissected and cut into rings (1.8-2.0 mm in length) under microscope, and were then mounted in a Multi Myograph system. The rings were stimulated with 100 nmol/L U46619, 30 nmol/L endothelin-1, or 60 mmol/L KCl to produce sustained contraction of the intrapulmonary arteries, after which resveratrol was applied cumulatively. Endothelium denudation, L-NAME and indomethecin were used to investigate the effect of resveratrol on constrictions of the isolated arteries, suing DMSO as the control.</p><p><b>RESULTS</b>Resveratrol induced concentration-dependent relaxations in endothelium-intact rings that contracted in response to stimulations with U46619, ET-1 and KCl, with pD2 of 3.82±0.20, 3.84±0.57, and 3.68±0.27, Emax of (99.58±0.83)%, 100%, and (99.65±0.98)%, respectively. Treatment of the arterial rings with the eNOS inhibitor L-NAME, but not with indomethecin or endothelium denudation, obviously affected the relaxant effects of resveratrol.</p><p><b>CONCLUSION</b>Resveratrol can concentration-dependently produce relaxant effect on human intrapulmonary arteries independent of the endothelium possibly by promoting synthesis and release of NO.</p>
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1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Artéria Pulmonar
/
Estilbenos
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Vasoconstrição
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Técnicas In Vitro
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Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Article