Expression of survivin, Fas, bcl-2 and bax in bone marrow cells from acute myeloid leukemia patients and its clinical significance / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 259-262, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-355669
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>UNLABELLED</b>To study the clinical significance of the expression of antiapoptosis gene, survivin and bcl-2, and proapoptosis gene, Fas and bax, in acute myeloid leukemia (AML), RT-PCR was used to examine the expression of survivin and flow cytometry (FCM) to detect the expression of Fas, bcl-2, bax and bcl-2/bax ratio in 68 cases of AML. The results demonstrated that (1) The positivity of survivin mRNA expression was significantly higher in AML compared to control (70.6% vs 30%, P < 0.05). (2) The expression of Fas and bcl-2 in AML before treatment was significantly higher than that in control (P < 0.001), but the bax expression did not (P > 0.05). (3) The survivin-positive AML cases showed a significantly lower Fas and higher bcl-2 expression in comparison with survivin-negative ones (P < 0.01 and P < 0.001, respectively), but the bax did not (P > 0.01). (4) Survivin-positive AML cases had a lower CR rate as compared with survivin-negative cases (64.6% vs 90%, P < 0.05). (5) The survivin-positive CR cases showed a decreased expression of Fas and bcl-2 after treatment in comparison with pretreatment expression (P < 0.001), but the bax expression remained unchanged before and after therapy. The survivin-positive NR cases showed a significantly decreased Fas and increased bcl-2 expression as compared with pretreatment expression (P < 0.001). bcl-2/bax ratio was also significantly higher in NR cases.</p><p><b>IN CONCLUSION</b>70.6% AML cases showed positive for survivin expression with a lower CR rate, the survivin-positive AML showed a low Fas with high bcl-2 expression and bcl-2/bax ratio as compared to the survivin-negative cases.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Leucemia
/
Outras Neoplasias Malignas
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Células da Medula Óssea
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Leucemia Mieloide Aguda
/
Biomarcadores Tumorais
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Regulação Neoplásica da Expressão Gênica
/
Proteínas Proto-Oncogênicas
/
Receptor fas
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Usos Terapêuticos
/
Tratamento Farmacológico
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo