Experimental study on apoptosis in leukemia cells induced by econazole / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 391-393, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-356552
Biblioteca responsável:
WPRO
ABSTRACT
To investigate apoptosis of mouse leukemia cell (WEHI-3) induced by econazole and its mechanism, apoptosis induced by econazole was examined by flow cytometry, while free calcium ([Ca(2+)]i) was determined by Fura-2 fluorescein load technique. The protein was isolated from endoplasmic reticulum of WEHI-3 cells, and then the expression of caspase-12 and caspase-7 was evaluated by Western blot. The results showed that WEHI-3 exhibited typical change of apoptosis when it was treated by econazole, [Ca(2+)]i was significantly higher in comparison with the control. The expression of caspase-12 and caspase-7 enhanced as the econazole concentration increased. In conclusion, econazole can induce WEHI-3 cell apoptosis and the caspase-12 plays a key role in this process.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Leucemia
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Leucemia
/
Western Blotting
/
Cálcio
/
Apoptose
/
Linhagem Celular Tumoral
/
Relação Dose-Resposta a Droga
/
Econazol
/
Caspase 7
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo