Detection of Epstein-Barr virus DNA in plasma/serum: a useful serological indicator for diagnosis of nasopharyngeal carcinoma / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1895-1897, 2002.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-356882
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the detection rates of Epstein-Barr virus (EBV) DNA in the serum/plasma between apparently healthy adults (AHAs) and nasopharyngeal carcinoma (NPC) patients in attempt to evaluate the efficiency of EBV DNA assay for serodiagnosis of NPC.</p><p><b>METHODS</b>The plasma and serum were obtained from 58 AHAs and 66 untreated NPC patients. EBV DNA W-fragment was detected using nested ploymerase chain reaction (PCR). Immunoenzymatic assay for titration of IgA-VCA was also adopted.</p><p><b>RESULTS</b>EBV DNA detection rate (84.85%) in the plasma/serum of 66 NPC patients was significantly higher than that (10.34%) in 58 AHAs. The sensitivity of plasma/serum EBV DNA assay (0.8485) was higher than that (0.8030) of titrating IgA-VCA (positive criterion >/= 140) though the specificities of these two tests were the same (0.8966). The correct rate, predictive value of a positive test, and Odds ratio of dual positivity (0.8387, 0.9792 and 141.0, respectively) were higher than those of single positivity either to plasma/serum EBV assay (0.5242, 0.7333 and 1.1423, respectively) or to IgA-VCA >/= 140 test (0.4839, 0.5385 and 1.0480, respectively).</p><p><b>CONCLUSION</b>The EBV DNA detection in the plasma/serum using nested PCR may be a useful indicator for serodiagnosis of NPC.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Sangue
/
DNA Viral
/
Imunoglobulina A
/
Testes Sorológicos
/
Neoplasias Nasofaríngeas
/
Herpesvirus Humano 4
/
Diagnóstico
/
Antígenos Virais
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2002
Tipo de documento:
Artigo