Expression of plasma miR-106a in colorectal cancer and its clinical significance / 南方医科大学学报
Journal of Southern Medical University
; (12): 354-357, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-356922
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect plasma miR-106a level in patients with colorectal cancer (CRC) and analyze its correlation to the clinicopathological features and disease diagnosis.</p><p><b>METHODS</b>miRNA expression profiling was performed using miRNA microarray chip for 3 colorectal adenocarcinoma samples and matched normal tissues. Plasma samples was collected from 50 colorectal cancer patients for quantitative analysis of miR-106a using real-time RT-PCR using 47 plasma samples from healthy volunteer as the control. Forty plasma samples were collected from these patients 7 days after operation to examine the changes in miR-106a expression.</p><p><b>RESULTS</b>miR-106a was differentially expressed in colorectal adenocarcinoma compared to normal tissues. The plasma levels of miR-106a expression were significantly higher in the cancer patients than in the healthy control group (P=0.012). miR-106a expression significantly decreased after the operation compared with its preoperative level (P<0.01), and no correlation was found between preoperative plasma miR-106a and the clinicopathological features including lymph node metastasis and TNM stage (P>0.05). miR-106a showed a receiver operating characteristic (ROC) curve area of 66.1%, a sensitivity of 62.3%, and a specificity of 68.2% in discriminating colorectal cancer patients from the control subjects.</p><p><b>CONCLUSION</b>plasma miR-106a is up-regulated in CRC patients, suggesting its potential value for the diagnosis of CRC.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Neoplasias Colorretais
/
Outras Neoplasias Malignas
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
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Neoplasias Colorretais
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Estudos de Casos e Controles
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Regulação para Cima
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Sensibilidade e Especificidade
/
MicroRNAs
/
Diagnóstico
/
Metástase Linfática
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
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Estudo prognóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo