Utilizing 2-DE and MALDI-TOF MS/MS to screen differentially expressed serum proteins of silicosis / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 136-141, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-357578
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish 2-dimensional gel electrophoresis (2-DE) images and seek differentially expressed serum proteins for understanding the pathogenesis of silicosis.</p><p><b>METHODS</b>2-DE and matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI-TOF-MS/MS) were used to screen differentially expressed serum proteins among silica-exposed population, suspect of silicosis (0+), phase one (I) group with silicosis and control group(non silica exposure).</p><p><b>RESULTS</b>Complement C4, leucine-rich alpha-2-glycoprotein and alpha-1-antitrypsin were significantly highly expressed in suspect of silicosis (0+) group(P < 0.01), but lowly in other groups. Inversely, serotransferrin was significantly down-regulated only in suspect of silicosis (0+) group(P < 0.01). Plasma glutathione peroxidase, tetranectin, apolipoprotein A-I and transthyretin were equally expressed in the serum of control group and silica-exposed population group, but decreased in the suspect of silicosis (0+) and phase (I) group.</p><p><b>CONCLUSION</b>Complement C4, leucine-rich alpha-2-glycoprotein, alpha-1-antitrypsin, serotransferrin, plasma glutathione peroxidase, tetranectin, apolipoprotein A-I and transthyretin are differentially expressed in the silica-exposed group and phase (I) group with silicosis, and the result should be validated by other biochemical technologies.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Silicose
/
Sangue
/
Proteínas Sanguíneas
/
Eletroforese em Gel Bidimensional
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Proteômica
/
Métodos
Limite:
Adulto
/
Idoso
/
Idoso, 80 anos ou mais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2007
Tipo de documento:
Artigo