Mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 401-403, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-358620
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells.</p><p><b>METHODS</b>MTT assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment.</p><p><b>RESULTS</b>Capsaicin induced A375-S2 cell death in a time- and dose-dependent manner. Sub-diploid peak was seen at 24 h after 250 micromol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase (ICAD) was reduced with the lapse of time.</p><p><b>CONCLUSION</b>Capsaicin induces A375-S2 cell apoptosis and down-regulation of ICAD contributes to this process.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Melanoma
/
Doenças da Pele
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias Cutâneas
/
Capsaicina
/
Transdução de Sinais
/
Apoptose
/
Caspases
/
Linhagem Celular Tumoral
/
Relação Dose-Resposta a Droga
/
Proteínas Reguladoras de Apoptose
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2005
Tipo de documento:
Artigo