Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1 / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the regulation effects of upstream stimulatory factor 1 (USF1) on osteopontin expression in odontoblasts.</p><p><b>METHODS</b>Odontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed.</p><p><b>RESULTS</b>Clones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group.</p><p><b>CONCLUSIONS</b>Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF.</p>