Establishment of a modified rabbit model of acute lung injury induced by cardiopulmonary bypass / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish an modified rabbit model of the acute lung injury induced by cardiopulmonary bypass (CPB) with ascending aorta and right atrium catheterization and detect the changes in serum tumor necrosis factor-α (TNF-α) level after modeling.</p><p><b>METHODS</b>Ten healthy adult male New Zealand rabbits were randomly selected to establish CPB models. The model establishment was deemed successful if the rabbits survived for over 4 h with stable heart beat after termination of CPB. The vital signs of the rabbits were recorded after anesthesia (T), before CPB (T), at 15 after blocking the ascending aorta and pulmonary artery (T), immediately after re-opening of the ascending aorta and pulmonary artery (T), and at 1 (T) and 4 (T) after CPB. Arterial blood gas (ABG) was monitored at T, Tand Tand the serum levels of TNF-α were also detected with ELISA.</p><p><b>RESULTS</b>Nine rabbit models of CPB with acute lung injury were successfully established. During the operation, the MAP was maintained at a level above 55 mmHg, HCT significantly decreased from (30.18∓2.88)% at Tto (17.73∓1.95)% at T(P<0.05), and plasma lactate level increased significantly from 3.65∓1.13 mmol/L at Tto 9.36∓1.28 mmol/L at T(P<0.05). The oxygenation index (PaO/FiO) at Twas significantly lower than that at T(281.64∓55.76 vs 468.36∓56.28 mmHg, P<0.05). The serum levels of TNF-α were significantly increased (P<0.05) and obvious lung interstitial edema and inflammatory cell infiltration occurred after CPB establishment.</p><p><b>CONCLUSION</b>The modified rabbit model of CPB with acute lung injury is stable and reliable and can be used for studying acute lung injury induced by CPB.</p>