Rapid detection of rpoB gene mutations in rif-resistant M. tuberculosis isolates by oligonucleotide microarray / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 253-258, 2009.
Article
em En
| WPRIM
| ID: wpr-360668
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray.</p><p><b>METHODS</b>Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray.</p><p><b>RESULTS</b>Of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes.</p><p><b>CONCLUSION</b>Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Rifampina
/
Proteínas de Bactérias
/
RNA Polimerases Dirigidas por DNA
/
Regulação Bacteriana da Expressão Gênica
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Farmacorresistência Bacteriana
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Genética
/
Metabolismo
/
Antibióticos Antituberculose
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2009
Tipo de documento:
Article