The Role of ICAM-1 and IL-8 in Reperfusion Injury Following Acute Arterial Occlusion of the Extremities / 日本心臓血管外科学会雑誌

ABSTRACT

A model of reperfusion injury following acute arterial occlusion was made in Wistar strain rat. Using this model, changes in and the role of interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) were estimated. The rats were divided into two groups as follows; the infra-renal aorta and the bilateral common femoral artery were ligated for six hours and released (reperfusion group, <i>n</i> 4), and only preparation of these arteries with no clamp was performed in the control group (<i>n</i> 4). CPK and IL-8 were measured, and various organs (heart, lung, kidney, femoral artery and carotid artery) were extracted, then ICAM-1 was evaluated immunohistochemistrically, using anti-rat ICAM-1 antibody (1A29). CPK showed a significantly high value in the reperfusion group. After the reperfusion, IL-8 increased significantly and remained high in the reperfusion group. Immunohistologically, the manifestation of ICAM-1 was recognized in the various organs of the reperfusion group. These results suggests that the high IL-8 values and the manifestation of ICAM-1 were caused by disorders in various organs by neutrophils, and these mechanisms may be related to the incidence of myonephropathic metabolic syndrome (MNMS), which is caused by acute arterial occlusion disease.