Detection of hepatitis B virus YMDD variants using a matrix-assisted laser desorption time of flight mass spectrometric analysis / 中国医师杂志
Journal of Chinese Physician
; (12): 1591-1595, 2008.
Article
em Zh
| WPRIM
| ID: wpr-397109
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the value of detecting hepatitis B virus (HBV) YMDD variants by matrix-assisted laser de-sorption time of flight mass spectrometry (MALDI-TOF MS). Methods The assay is based on polymerase chain reaction (PCR) amplifica-tion and mass measurement of oligonucleotides containing sites of mutation of the YMDD motif. Result The MALDI-TOF MS-based genoty-ping assay was sufficiently sensitive to detect as few as 100 copies of HBV genome per milliliter of serum, and this method had superior spe-cificity for determining mixtures of wild-type and variant viruses. When sera of 40 patients were analyzed, the MALDI-TOF MS-based assay correctly identified known viral variants and additional viral quasi-species not detected by previous methods, as well as their'relative abun-dance. Conclusion The sensitivity, specificity and amenability to high-throughput analysis make MALDI-TOF MS-based assay suitable for mass screening of HBV infected patients who are receiving lamivudine.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Journal of Chinese Physician
Ano de publicação:
2008
Tipo de documento:
Article