Neuron apoptosis in response to hyperoxic liquid in rabbit with traffic brain injury / 中华创伤杂志

ABSTRACT

Objective To investigate the effect of hyperoxie liquid on neuron apoptosis in rabbit with traffic brain injury. Methods Rabbit models of brain injury were established by screening suit-able striking intensity and supplied respectively with hyperoxic liquid for 25, 50, 75 and 100 ml each day to observe the respiratory control rate(RCR) of mitochondria, mitochondria membrane potential (MMP), apoptosis rate of neurocytes and NSS grade.Meanwhile, non-intervention group and control groups were set.Results The rabbit model made by striking velocity of 72 km/h was selected as the study object.MMP, RCR, phosphate/oxygen ratio(P/O), oxygenated phosphate rate(OPR) and apopto-sis rate of neurocytes and NSS grade in control and treatment group showed statistical difference compared with non-intervention group(P<0.05).MMP, RCR, P/O and OPR in 100 ml hyperoxic liquid treat-ment group were higher than those in 75 ml hyperoxie liquid treatment group(P<0.05), but the apopto-sis rate of neurocytes and NSS grade showed no statistical difference(P>0.05). Conclusion Intra-venous hyperoxic liquid infusion can decrease apoptosis rate of neurocytes, as indicates that hyperoxic liq-uid affects apoptosis of neurocytes and hence ameliorates prognosis mainly by intervening respiratory func-tion of mitechondria.