Study on the mechanisms of T cell vaccination-induced survival prolongation of cardiac allograft in rats / 中华器官移植杂志

ABSTRACT

Objective To investigate the effect of T cell vaccination(TCV)on the survival time of cardiac allograft in rat.Methods Rat heterotopic cardiac transplantation was performed,Donor antigenprimed recipients' spleen cells were obtained bv 1/3 splenectomy for the preparation of TCV.TCV was then intraperitoneally into the abdominal cavity again.The survival time of cardiac allograft was observed.Results TCV could markedly prolonged rat cardiac allograft survival.T cell proliferation was increased and B cell proliferation was not affected in the vaccinated rats.The mixed lymphocyte reaction(MLR)was inhibited,The analysis of phenotypes on T cells showed that the number of CD8+ clones in vaccine cells and vaccinated rat spleen cells were increased.The antibody-mediated cytotoxicity was not affected by TCV.Conclusions TCV enhances the recipients' T cell-mediated immune response.The TCV-induced hyporeactivity to specific donor antigen is related to the induction of anti-idiotypic response and is not associated with humoral mechanisms.