PrP 106-126 Altered PrP mRNA Gene Expression in Mouse Microglia BV-2 Cells' / 中国病毒学·英文版
Virologica Sinica
; (6): 440-444, 2010.
Article
em Zh
| WPRIM
| ID: wpr-402286
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WPRO
ABSTRACT
Prion diseases are infectious and fatal neurodegenerative diseases. The pathogenic agent is an abnormal prion protein aggregate. Microglial activation in the centre nervous system is a characteristic feature of prion disease. In this study, we examined the effect of PrP 106-126 on PrP mRNA gene expression in Mouse microglia cells BV-2 by real-time quantitative PCR. PrP mRNA expression level was found to be significantly increased after 18 h exposure of BV-2 cells to PrP 106-126, with 3-fold increase after 18 h and 4.5-fold increase after 24 h and BV-2 cells proliferating occurred correspondingly. Our results provide the first in vitro evidence of the increase of PrP mRNA levels in microglial cells exposed to PrP 106-126, and indicate that microglial cells might play a critical role in prion pathogenesis.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Virologica Sinica
Ano de publicação:
2010
Tipo de documento:
Article