Inhibitory effect of EGCG on proliferation and HIF-1α/VEGF expression in cell line HepG2 / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 713-720, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-403040
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To study the molecular mechanism of EGCG on inhibiting the growth of hepatic carcinoma.METHODS:
The proliferation of hepatic cell line HepG2 cultured with different doses of EGCG was studied by MTT and suspension/adherence methods. The effect of EGCG on the expression of HIF-1α/VEGF at mRNA and protein levels in vitro and in vivo was evaluated by RT-PCR and Western blotting, respectively. The inhibition of EGCG on the growth of tumor implanted into athymic nude mice was also observed.RESULTS:
The proliferation of hepatic cell line HepG2 was inhibited by EGCG in a dose-dependent manner. The expression of HIF-1α/VEGF was suppressed markedly by EGCG at protein level. However, the inhibitory effect of EGCG on the mRNA expression was only observed on VEGF, not on HIF-1α. In the animal experiment, the implanted tumor growth was inhibited by 39.8%±5.1%.CONCLUSION:
EGCG suppresses the hepatic carcinoma cell growth, and interrupts the HIF-1α/VEGF signaling pathway significantly, indicating a fundamental mechanism of EGCG for inhibiting tumor growth.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2010
Tipo de documento:
Artigo