Expressing of bovine viral diarrhea-mucosal disease virus Changchun184 strain of E2 gene in BCG / 中国兽医学报
Chinese Journal of Veterinary Science
; (12): 854-857, 2009.
Article
em Zh
| WPRIM
| ID: wpr-406349
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WPRO
ABSTRACT
E2 gene of BVDV Changchun 184 strain was cloned and inserted into the shuttle expression plasmid vector pMV261,the recombinant shuttle plasmid pMV261-E2 was constructed.Then pMV261-E2 was transformed into BCG successfully and obtained recombinant BCG which was resistive to kanamyein.The recombinant BCG were identifieated by PCR.E2 gene expression in recombinant BCG was induced in 45℃,then the SDS-PAGE and western blotting was used to analyze the expression product.The results indicated the BVDV E2 gene was expressd in BCG successfully.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Veterinary Science
Ano de publicação:
2009
Tipo de documento:
Article