Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env / 中国病毒学·英文版
Virologica Sinica
; (6): 233-246, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-407087
Biblioteca responsável:
WPRO
ABSTRACT
Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around Nab epitopes and deletions of variable regions in env.The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γELISPOT.Overall,five mutants(dWt,M2,M5-2,M5-1 and dM7)induced highed neutralization activities for both pseudoviruses than plasmid Wt,while only two of the mutants(dWt and M5-2)showed significant differences(P<0.05).Two mutants(M2 and dM2)induced more Env-specific T cells than plasmid Wt.Statistically however,significance was only reached for mutant M2.Thus,properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Virologica Sinica
Ano de publicação:
2008
Tipo de documento:
Artigo