Research of Fusion Expression of Human β-Defensin-3 in Escherichia coli / 中国比较医学杂志
Chinese Journal of Comparative Medicine
; (6): 670-675, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-407569
Biblioteca responsável:
WPRO
ABSTRACT
Objective To achieve the fusion expression of the entire human beta-defensin-3(hBD-3) gene. Method We synthesized two oligonucleotide primers accor ding to the codon preference of Escherichia coli. The gene was cloned into p GEX -4T-2 to establish the pGEX-4T-2-hBD-3 as the fusion expression vector by PCR. Transformed into E.coli strain DH5α, the express vector was induced an d ex pressed by IPTG. The fusion protein GST-hBD-3 was obtained by repeated cycles of freezing and thawing, cut by thrombin to attain the recombinant hBD-3 protei n. Result The result of the antibacterial peptide agarose diffu sion assay shows the antibacterial activity of the rhBD-3 against the S.aureu s exists, and it reaches 0.843U. Conclusion The fusion expr ession of the hBD-3 gene is successful.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Comparative Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo