The genetic structure of the fibroblastic Fas in keloid

Yongbo LIU; Xiaojun LIU; Jianhua GAO; Hongjie DUAN

ABSTRACT

BACKGROUND:

Keloid is the outcome of wound-healing process,and the result of massive accumulation of life-prolonged fibroblasts with gene mutation as well as the excessive synthesis of collagenous fibers.

OBJECTIVE:

To probe into the structural relations of exons 7-9 of fibroblastic Fas gene in keloid tissues.

DESIGN:

A self-controlled experimental study with cicatricial tissues as the subjects.

SETTING:

Department of Plastic Surgery of Southern Hospital of the First Military Medical University of Chinese PLA.

PARTICIPANTS:

This experiment was carried out at the Tropical Disease Research Institute of the First Military Medical University of Chinese PLA in 2001. All keloid and hypertrophic scar tissues were obtained from the patients who received orthopedic surgical operations at the Southern Hospital, including 15 patients with keloid and 12 patients with hypertrophic Scars. Normal skin and peripheral blood were obtained from the keloid patients as self-control. Meanwhile pathological tissues and normal skin and peripheral blood were obtained from patients with hypertrophic scars as normal control.INTERVENTIONPCR-single strand conformation polymorphism was used to find the structure of the exons 7-9 in Fas gene in15 patients with keloid.MAIN OUTCOME

MEASURES:

The structure of the exons 7-9 in Fas gene.

RESULTS:

Heterozygous loss of Fas gene exon-8 was observed in all the 15 keloid patients, and 20% of them displayed an increase in exon-9 allele band.

CONCLUSION:

The genetic structure of Fas gene showed no mutation in hypertrophic scars, normal skin and the peripheral blood,but mutations were detected in exons-8 ,and -9 of Fas gene in keloid. This was closely related with the disfunction of its encoded proteins.