Volatile oil of flos magnoliae biondii pamp for the foot tumefaction and the expression of prostaglandin E2 in joint tissue in rats with adjuvant arthritis

Wenkui WANG; Yingjun SHEN; Yun QI; Jinxiang SONG

ABSTRACT

BACKGROUND:

The aim of present study is to demonstrate that volatile oil of flos magnoliae biondii pamp(VOMbp) possess anti-immunology inflammatory property.

OBJECTIVE:

To explore the effect of VOMbp on adjuvant arthritis in rats.

DESIGN:

Grade sampling,randomized controlled quantitative analysis.

SETTING:

Teaching and Researching Department of Pharmacology,Chengdu University of Traditional Chinese Medicine.MATERIALSThis study was carried out at the Teaching and Researching Department of Pharmacology,Chengdu University of Traditional Chinese Medicine from December to April in 1999. Totally 120 Wistar rats were involved.

METHODS:

Wistar rats were randomly divided into 4 groupsmodel control group, 0.28 mL/kg and 0.14 mL/kg VOMbp group,and 25 mg/kg dex-amethasone group. Allergy were induced in all rats through intra-cutaneous injection of Freund's adjuvant (0.1 mL/rat)at metatarsus so as to establish multiple arthritis model in rat. According to different dosage,the experiments were carried out in three batches with 10 rats in each group.Rats in the first batch were given VOMbp three days before inducing allergy for consecutwe 3 days,in order to observe the influence of VOMbp on adjuvant induced acute non-specific inflammatory;Rats in the second batch were given VOMbp on the 8th day sensitization allergy for consecutive 7days so as to explore the protective effect on secondary pathological changes;rats in the third batch were administratedon the 19th day after sensitization for consecutive 7 days for study in therapeutic effect on sec ondary pathological changes. Meanwhile foot tumefaction and the level of prostaglandin E2in ankle arthritis were observed.MAIN OUTCOME

MEASURES:

① Effect of VOMbp on the adjuvant induced acute non-specific inflammatory. ② Preventive effect on adjuvant arthritis. ③ Therapeutic effect on adjuvant arthritis.

RESULTS:

① VOMbp could significantly suppress the primary foot tume faction. ②Moreover VOMbp of preventive dosage could most significantly suppress the secondary arthritis tumefaction [(0.231±0.065)mL of 0.28 mL/kg group and ( 0.206±0.082 ) mL of 0.14 mL/kg group] ,accompanied by PG-E2 decrement in tumefaction tissues [(0.133 2±0.057 2), (0.138 5 ±0.042 6)OD278nm correspondingly].③ No obvious effect of VOMbp of therapeutic dosage on secondary inflammatory tumefatcion of arthritis could be observed in 0.14 mL/kg group,but obvious decrement of prostaglandin E2 could be observed in edema tissue;While 0.28 mL/kg VOMbp exerted most significant or significant influence on above parameters.

CONCLUSION:

VOMbp possess inhibiting effect on Freund's adjuvant induced primary foot tumefaction and secondary arthritis,with the preventive effect better than therapeutic effects.The therapeutic effect was proved better in higher dosage group.