Construction of cDNA library of the treated Changliver cell and quality analysis / 新乡医学院学报
Journal of Xinxiang Medical College
; (12): 1-4, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-410082
Biblioteca responsável:
WPRO
ABSTRACT
Objective To construct cDNA library of the treated Changliver cell by switching mechanism at 5′ end of RNA transcript (SMART) technique and analyze its quality.Methods cDNA of Changliver cell was aquired with reverse transcription polymerase chain reaction (RT-PCR) and long-distance PCR (LD-PCR),then the cDNA library was constructed with SMART cDNA library construction kit.Results Through testing,the high quality cDNA library containing full length cDNA of Changliver cell had been constructed.The titer of the amplified cDNA library was 4.5 × 1010 pfu*ml-1 and the average exogenous inserts of the recombinants was 1.5 kb.Conclusion These results suggest that the Changliver cell cDNA library has a high quality and lays a solid foundation for researching on Changliver cell and screening
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Xinxiang Medical College
Ano de publicação:
2004
Tipo de documento:
Artigo