Detection of anti-Sm antibodies in SLE serum by immuno-PCR / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 263-265, 2001.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-410406
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To apply immuno-PCR for detection of anti-Sm antibodies.Methods:
Immuno-PCR was established for anti-Sm antibodies in SLE sera using SaHIgG-DNA probe. The experimental conditions were optomized and methological identifieation indicated obvious in crease(×107 ) in sensitivity compared with that of ELISA. The positive rate of anti-Sm antibodies was 54.9% in SLE sera, and significantly higher than that of ELISA. Intra-and inter-CV were 2.3%~4.8% and 3.7%~6.9% respectively. Immuno-PCR was positively correlated with ELISA( p <0.001). Therefore, immuno-PCR for anti-Sm antibodies is specific,sensitive,reproducible and practical clinically.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2001
Tipo de documento:
Artigo