Effect of quateranary ammonium salt derivative (F2) of haloperidol on calcium in vascular smooth muscle cells / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 54-56, 2001.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-412078
Biblioteca responsável:
WPRO
ABSTRACT
AIM To observe the effect of quateranary ammonium salt derivative (F2) of haloperidol on calcium level in vascular smooth muscle cells (VSMCs) isolated from thoracic aortas of rat. METHODS VSMCs were loaded with Fluo-3-AM, a calcium sensitive fluorescent dye, and [Ca2+]i was determined by the use of laser scanning confocal microscope (LCSM). RESULTS In Ca2+ (1.26 mmol*L-1) bath solution, intracellular calcium fluorescent intensity (FI) in VSMCs was increased when application with 30 mmol*L-1 KCl, and then was inhibited after addition with F2. The FI of the ASMCs with different concentrations of F2 (0.1,1,10,100 μmmol*L-1)within three minutes were 64%±9%(n=16, P<0.05),16%±5%(n=20, P<0.01),0.6%±0.8%(n=20, P<0.01),0.1%±0.3%(n=15, P<0.01), respectively. CONCLUSION F2 could decrease the intracellular Ca2+ level in the VSMCs by blocking the voltage-dependent calcium channel.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2001
Tipo de documento:
Artigo