Quantitative Determination of Mangiferin in Rhizoma Belamcandae and Its Substitute of Iris L / 中草药

ABSTRACT

Mangiferin, one of the active constituents of Rhizoma Belamcandae, in samples of Be-lamcanda chinensis (L.) DC. or its substitute was determined quantitatively by RP-HPLC. The 11 sam-ples collected from different localities for analysis were 7 rhizomes of wildly grown or cultivated B. chi-nensis, 1 of its leaf and stem, and 3 substitutes (a wildly grown and another commercially available Iristectorum Maxim. and a I. dichotoma Pall. ). Results of the analysis showed that the contents of mangiferinin Rhizoma Belamcandae were significantly higher than that of its substitutes I. tectorum and I. di-chotoma. There were also certain significant differences between samples from different localities (P<0.05), but with no statistically significant difference between the rhizome or leaf and stem, neither be-tween cultivated and wildly grown samples, (P>0.05). The method was proved to be quick, simple andreproducible, and may provide a reliable basis for the quality control and evaluation of B. chinensis.