Effects of vascular endothelial growth factor from pulmonary stromal cells on differentiation and function of dendritic cells / 中华老年医学杂志
Chinese Journal of Geriatrics
; (12): 1043-1046, 2011.
Article
em Zh
| WPRIM
| ID: wpr-417539
Biblioteca responsável:
WPRO
ABSTRACT
Objective To observe the effects of vascular endothelial growth factor(VEGF)from pulmonary stromal cells on differentiation and function of mature dendritic cells (mDC) and the immune tolerance. Methods Co-culture system of murine pulmonary stroma cells (MPSC)/mDC was established as control group,and VEGF-Ab add-on treatment to co-culture cells as experimental group.Cytokines secreted from pulmonary stroma cells were detected by RT-PCR.The expression of cellular phenotype and ability of the mDC-induced T cells reproductive activity were detected by flow cytometry and CCK-8,respectively.Results CD86 expression of VEGF-Ab group was higher than of control group (P < 0.05).There was no difference in the ability of mDC-induced T cells reproductive activity between the two groups(P>0.05).Conclusions VEGF might be involved in the regulation of immune tolerance by reducing expression of CD86 in dendritic cells.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Geriatrics
Ano de publicação:
2011
Tipo de documento:
Article