Influence of cigarette smoking on inflammatory factor in sputum after inhaled corticosteroid treatment in asthma / 中国综合临床

ABSTRACT

Objective To investigate the impact of cigarette smoking on inflammatary factors in sputum including cell component,IL-8 and eotaxin,and the effect on responses to treatment with inhaled corticosteroids (ICS)in patients with asthma.Methods Thirty-eight outpatients with chronic stable asthma who visited from January 2009 to February 2010 were enrolled in the study.Twenty-three cases were nonsmokers and 15 cases were smokers.All of them were treated by daily inhaled budesonide,and[32 agonist when necessary.Induced sputum eosinophil and neutrophil proportion were measured,and the levels of interleukin(IL-8)and eotaxin in induced-sputum by enzymatic immunoassay(ELISA)were compared between non-smoking and smoking asthmatic patients.Results The eosinophil proportion was(2.0 ± 0.4)％ in smokers and(4.6 ± 2.5)％ in nonsmokers before therapy,and(1.1 ± 0.5)％ in smokers and(1.8 ± 0.6)％ in nonsmokers after therapy.The difference was statistical significant(F =15.271,P ＜ 0.05).The eotaxin was(3.5 ± 2.1)× 10-3 mg/L insmokers and(8.6 + 2.3)x 10-3 mg/L in nonsmokers before therapy,and(3.1 + 1.5)x 10-3 mg/L insmokers)and(3.6 ± 1.3)x 10-3 mg/L in nonsmokers after therapy.There was statistical significant(F =24.172,P ＜ 0.05).There were no significant difference on neutrophil proportion and IL-8 before and after treatment(F =1.563 and 1.793,respectively,Ps ＞ 0.05).Neutrophil proportion(F =9.632,P ＜ 0.05)and IL-8(F =5.720,P ＜ 0.05)in smokers were significantly higher than non-smokers,whereas eosinophil proportion (F =15.879,P ＜ 0.05)and eotaxin(F =12.365,P ＜ 0.05)in smokers were significantly lower than nonsmokers.Conclusion There was a significantly increase in inflammatary cells andfactors in induced-sputum in two groups after ICS treatment,but the effect in smokers was lower than in non-smokers.Neutrophil proportion and IL-8 were higher in induced sputum in smokers,whereas eosinophil proportion and eotaxin were higher in non-smokers.