Effect of momordin in inhibiting proliferation and inducing apoptosis of multidrug-resistant K562/A02 cells and its molecular mechanism / 肿瘤
Tumor
; (12): 288-292, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-433346
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To study the molecular mechanism for momordin in inducing apoptosis of multidrug-resistant human chronic leukemia K562/A02 cells.Methods:
The growth inhibition value of K562/A02 cells was detected by CCK-8 method. Cell apoptosis was analyzed by Annexin Ⅴ flow cytometry (FCM) and cell morphological examination. FCM was also used in determining expression of P-glycoprotein, p53 protein, bcl-2 protein and caspase activity.Results:
Momordin inhibited the proliferation of K562/A02 cells in a dose-dependent manner. It also induced cell apoptosis, reduced the expression of P-glycoprotein, p53 protein and bcl-2 protein, and increased caspase-3 and caspase-8 activity.Conclusion:
Momordin reversed the inhibition of apoptosis in multidrug-resistant K562/A02 cells. The molecular mechanism may be related with down-regulation of expression of p53 protein, P-glycoprotein, and bcl-2 protein and up-regulation of caspase-3 and caspase-8 activities.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Tumor
Ano de publicação:
2010
Tipo de documento:
Artigo