Expression of IL-18 in the peripheral blood mononuclear cells of chronic hepatitis B patients / 免疫学杂志
Immunological Journal
; (12): 125-127, 2001.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-433876
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigated the possible roles of IL-18 in HBV infection, and the expression of IL-18 in the peripheral blood mononuclear cells (PBMC) of chronic hepatitis B. Methods In 15 cases of asymptomatic carriers, 30 cases of active and remissive phases of chronic hepatitis and 10 healthy individuals (as normal controls), IL-18 expression in PBMC were quantitatively analyzed with flow cytometric immunological method. The PBMC were separated routinely and stimulated with LPS (SIGMA) and Monensin (SIGMA) for 6 hours. Then the cells were harvested and fixed by PBS/4% paraformaldehyde. The treated cells were stored in liquid nitrogen for detection later. After immunologic stain, the expression of IL-18 in PBMC were examined by flow cytometry. Results ① IL-18 was the lowest in asymptomatic carriers. It was fewer in remissive phase than in normal controls (P<0.01).There was no difference between active phase and normal controls(P=0.25).②Within the group of active phase, IL-18 was significantly different between each grade of inflammatory activity(P<0.01),and correlated with serum ALT positively(r=0.63,P<0.01). Conclusion IL-18 may relate to disease activity and the inflammation of liver.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Immunological Journal
Ano de publicação:
2001
Tipo de documento:
Artigo