Change of intracellular calcium concentration in prostate smooth muscle cells of SD rats with chronic abacterial prostatitis under high potassium solution / 中华泌尿外科杂志
Chinese Journal of Urology
; (12): 224-228, 2013.
Article
em Zh
| WPRIM
| ID: wpr-434948
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the change of intracellular calcium ion concentration in prostate smooth muscle cells of SD rats with chronic abacterial prostatitis under high potassium solution.Methods SD rats were divided into experiment group and control group.The CP model was set up by castration and estradiol injection.The PSMC was cultured and purified in vitro.Laser confocal scanning microscope was used after the ceils were incubated with Quest Fluo-8TM.The cells were treated with high potassium solution,and the change of fluorescence intensity was observed.Results The pathologic specimens of the experiment group showed typical pathologic characteristics of chronic prostatitis under light microscope,the control group without inflammation performance.Using immunocytochemistry method confirmed that the experiment group and the control group were prostate smooth muscle cells.The change of fluorescence intensity of [Ca2+] i in the experiment group and control group in the high potassium solution was 27.86 ± 9.88 and 7.61 ± 4.31.There were statistically significant differences between the two groups (P < 0.01).Conclusions High potassium solution cause intracellular calcium ion concentration increased.
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1
Base de dados:
WPRIM
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Urology
Ano de publicação:
2013
Tipo de documento:
Article