Relationship between Nrf2-ARE pathway and acute lung injury induced by endotoxic shock in rabbits / 中华麻醉学杂志
Chinese Journal of Anesthesiology
; (12): 334-337, 2014.
Article
em Zh
| WPRIM
| ID: wpr-451170
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WPRO
ABSTRACT
Objective To evaluate the relationship between erythroid 2-related factor (Nrf2 )-antioxidant response element (ARE) pathway and acute lung injury (ALI) induced by endotoxic shock in rabbits .Methods Thirty healthy male New Zealand white rabbits ,aged 2 months ,weighing 1.5-2.0 kg ,were randomly divided into 3 groups (n=10 each) using a random number table :control group (group C) ,group ALI and all-trans retinoic acid group (group ATRA ) .In group ATRA ,all-trans retinoic acid 6 mg/kg (in filter sterilized vegetable oil 1.2 ml) was injected intraperitoneally once a day for 2 days .ALI was induced by lipopolysaccharide 5 mg/kg (in normal saline 2 ml ) injected via the auricular vein at 10 h after the last injection of ATRA in ALI and ATRA groups .The equal volume of normal saline was injected instead in group C .The rabbits were sacrificed at 6 h after lipopolysaccharide or normal saline administration .The pulmonary specimens were removed for determination of wet/dry lung weight ratio (W/D ratio ) and expression of Nrf2 mRNA and nuclear protein ,and HO-1 mRNA and protein in lung tissues .The pathological changes of lungs were scored .Results Compared with group C ,the pathological score and W/D ratio were significantly increased , and the expression of Nrf2 mRNA and nuclear protein ,and HO-1 mRNA and protein was up-regulated in ALI and ATRA groups ( P 0.05 ) .Conclusion Activation of Nrf2-ARE pathway is the regulatory mechanism of the body adapting to the ALI induced by endotoxic shock in rabbits .
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Anesthesiology
Ano de publicação:
2014
Tipo de documento:
Article