The effect of two different odontoblast inducer on the proliferation and apoptosis of rat adiposed-derived stem cells / 重庆医学
Chongqing Medicine
; (36): 881-884, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-460487
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the effect of two different odontoblast inducer on the proliferation and apoptosis of rat adiposed-derived stem cells.Methods Adiposed-derived stem cells were collected by enzyme digestion from inguinal fat pads of 4 days post natal mice.Immunocytochemistry was performed to identify the cells.MTT and flow cytometry were tested the prolifera-tion and apotosis of adiposed-derived stem cells by co-cultured with tooth germ cell conditioned medium(TGC-CM)or dentin non-collagenous protein medium (DNCPM).Results Cells displayed a fibroblast-like appearance and positively expressed CD44 and CD105 when cufured to the secend yeneration.After 3 day the cells polarity changed by co-cultured.Count of cells were no obvious change by TGC-CM co-cultured,while that ruduced significantly by DNCPM co-cultured.It confirmed that the proliferation rate of ADSCs in TGC-CM group and control group is higher than DNCPM group(P 0.05).Conclusion TGC-CM may have more advantage as inducer in rat adiposed-derived stem cells differentiate into dentin like cells than DNCPM.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chongqing Medicine
Ano de publicação:
2015
Tipo de documento:
Artigo