PLCE1 suppresses apoptosis of lung adenocarcinoma cells via modulating p53 expression / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 119-123, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-474660
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To investigate the role of phospholipase C epsilon 1 ( PLCE1 ) in modulating the apoptotic mechanism in lung adenocarcinoma A 549 cells.METHODS:
PLCE1 inhibitor U-73122 was used to suppress the expres-sion of PLCE1.The expression of PLCE1 and p53 in A549 cells was evaluated by quantitative real-time PCR and Western blotting.Apoptosis was assessed by flow cytometry .RESULTS:
A549 cells expressed high level of PLCE1 and low level of p53.Inhibition of PLCE1 markedly increased the expression of p 53, and increased the apoptosis of A 549 cells.CON-CLUSIONPLCE1 suppresses apoptosis of A549 cells via inhibiting the expression of p53.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2015
Tipo de documento:
Artigo