The protection of ethanol extract of Zingiber officinale against focal hepatic ischemia-reperfusion injury in rats / 国际中医中药杂志

ABSTRACT

Objective To investigate the protective effects of the ethanol extract of Zingiber officinale against focal hepatic ischemia-reperfusion injury in rats and itspossible mechanism.Methods The rats were randomly divided into six groups sham operation group, model control group, ethanol extract of Zingiber officinale 100, 200, 400 and 600 mg/kg per-treated groups. The rats in the sham operation group and the model control group were given equal volume of saline by intragastric administration, once a day; two weeks later, the model were made. Except the sham operation group, the focal hepatic ischemia-reperfusion rats models were established in other groups. Two hours after ischemic-reperfusion, the activity of SOD/GSH-Px and the content of MDA in hepatic tissue were determined; the contents of ALT, AST and LDH in serum were determined; the histopathological changes and hepatocyte apoptosis were observed.Results Compared with the model control group, the activity of SOD in hepar tissue of Ethanol extract of Zingiber officinale 100, 200, 400 and 600 mg/kg per-treated groups (7.37 ± 1.89 U/mg, 10.18 ± 2.42 U/mg, 10.50 ± 2.31 U/mg, 11.17 ± 2.83 U/mgvs. 5.61 ± 1.72 U/mg) were significantly increased (P<0.05,P<0.01), the activity of GSH-Px (11.31 ± 2.64 U/mg, 13.07 ± 3.72 U/mg, 14.63 ± 3.40 U/mg, 15.14 ± 3.81 U/mgvs. 10.92 ± 2.25 U/mg) were significantly increased (P<0.05,P<0.01), and the content of MDA (8.53 ± 2.05 nmol/mg, 5.44 ± 1.37 nmol/mg, 5.20 ± 1.18 nmol/mg, 4.96 ± 1.42 nmol/mgvs. 10.26 ± 2.27 nmol/mg) were significantly decreased (P<0.05,P<0.01); the content of ALT in serum of the ethanol extract of Zingiber officinale 100, 200, 400 and 600 mg/kg per-treated groups (974.62±341.53 U/L, 621.83 ± 214.37 U/L, 553.75 ± 173.48 U/L, 517.92 ± 204.26 U/Lvs. 1 429.27 ± 618.33 U/L), AST (624.17 ± 148.62 U/L, 456.83 ± 127.35 U/L, 407.54 ± 141.49 U/L, 361.90 ± 104.73 U/Lvs. 957.61 ± 346.82 U/L), LDH (1 082.49 ± 312.35 U/L, 897.33 ± 242.76 U/L, 828.42 ± 265.84 U/L, 776.58 ± 182.47 U/Lvs. 1 385.61 ± 543.74 U/L) were all significantly decreased(P<0.05,P<0.01); and the histopathological changes and the hepatocyte apoptosis were significantly improved compared with the model control group.Conclusions The ethanol extract of Zingiber officinale could effectively reduce the damage of free radical, improve hepatic function, inhibit the histopathological changes and hepatocyte apoptosis, suggesting that the ethanol extract of Zingiber officinale had protective effects against focal hepatic ischemia-reperfusion injury in rats.