Celastrol induces caspase-dependent apoptosis through ROS/JNK path-way in Saos-2 cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 1457-1461, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-477354
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
ToexploretheeffectofcelastrolonapoptosisofSaos-2cellsanditsmechanism.METH-ODSSaos-2 cells were treated with various concentrations of celastrol , and the cell viability was measured by MTT assay . Apoptosis and reactive oxygen species ( ROS) production were determined by flow cytometry .The protein levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated JNK were evaluated by Western blot .RESULTS:
The viability of Saos-2 cells was significantly inhibited by celastrol .Celastrol significantly induced apoptosis of Saos-2 cells.Celastrol signifi-cantly induced ROS production in the Saos-2 cells.Western blot analysis demonstrated that celastrol significantly increased the protein levels of cleaved caspase-9, cleaved caspase-3 and phosphorylated JNK in the Saos-2 cells.CONCLUSION:
Celastrol induces caspase-dependent apoptosis through ROS/JNK pathway in Saos-2 cells.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2015
Tipo de documento:
Artigo