Protective effect of hydrogen sulfide on pressure ulcer rats with ischemia/reperfusion injury and its mecha-nisms / 中华实用儿科临床杂志

ABSTRACT

Objective To explore the protective effect and mechanism of exogenous hydrogen sulfide ( H2 S) on pressure ulcer rats with ischemia/reperfusion ( I/R) injury. Methods Forty SD rarts were randomly divided into 4 groups,namely normal control group,I/R model group,low-dose H2 S intervention group and high-dose H2 S interven-tion group. Each group had 10 animals. The low-and the high-dose H2 S intervention groups were injected intraperitone-ally 10 and 30μmol/kg of sodium hydrosulfide ( NaHS) for 1 week,respectively;however,the normal control group and I/R model group were treated with same volume 9 g/L saline. Then,the normal control group did not sustain any pres-sure,but other 3 groups were pressurized. After 3 circulations of ischemia/reperfusion,all rats were sacrificed,and path-ological changes in pressure ulcer rats were evaluated by HE staining. Serum H2 S contents were analyzed through meth-ylene spectrophotometric method. Serum levels of tumor necrosis factor-α(TNF-α),interleukin-6 (IL-6),intercellu-lar adhesion molecule -1 (ICAM-1),and myeloperoxidase (MPO) were detected by enzyme linked immunosorbent assay ( ELISA ) . The activities of malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) , glutathione-peroxidase ( GSH-Px) and catalase ( CAT) in the homogenate of muscle were examined. Apoptotic index ( AI) was calculated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling ( TUNEL) . The expressions of Bcl-2 and Bax in muscle tissue were evaluated by Western blot. Results In comparison with the normal control group,serum TNF-α, IL-6,ICAM-1 and MPO levels,MDA activity in the homogenate of muscles,AI and Bax expression level were in-creased (all P<0. 01),while serum H2S levels,the activities of SOD,GSH-Px and CAT as well as Bcl-2 expression levels were reduced in the I/R model group (all P<0. 01). Upon intervention therapy of low-or high-dose H2S,path-ological scores,serum TNF-α,IL-6,ICAM-1 and MPO levels,MDA activity in the homogenate of muscle,AI and Bax expression levels were gradually reduced while serum H2 S levels,the activities of SOD,GSH-Px and CAT and Bcl-2 expression levels were gradually enhanced when compared to those of the I/R model group (all P<0. 05,0. 01). Con-clusions Exogenous H2 S has better protection against pressure ulcer rats with I/R injury. The mechanism may be as-sociated with inhibition of inflammatory reaction and enhancement of antioxidative ability as well as reduction of apopto-sis.